N-ACETYLCYSTEINE DOES NOT IMPROVE SPERM MOTILITY OF LIDIA BULL AFTER PROLONGED EPIDIDYMAL STORAGE / LA N-ACETILCISTEINA NO MEJORA LA MOTILIDAD ESPERMÁTICA EN TOROS DE LIDIA LUEGO DEL ALMACENAMIENTO EPIDIDIMARIO PROLONGADO

ELVIRA MATILLA PINTO, LAURO GONZÁLEZ-FERNÁNDEZ, NURIA HERNÁNDEZ-ROLÁN, CAROLINA TOBAJAS VEGA, VIOLETA CALLE-GUISADO, JOSÉ MIJARES-GORDÚN, FRANCISCO MIGUEL SÁNCHEZ MARGALLO, IGNACIO SANTIAGO ÁLVAREZ MIGUEL, BEATRIZ MACIAS-GARCÍA

Resumen


The Lidia bovine breed is considered a hallmark of Spanish cattle industry. Assisted reproductive techniques like cryopreservation of epididymal spermatozoa could be considered as an important tool to obtain more offspring and store its genetics. As these bulls are not selected by their reproductive performance or sperm freezability, the quality of their ejaculates is poor and addition of antioxidants prior cryopreservation could exert beneficial effects on the post-thaw sperm quality. The aim of this study was to evaluate the effect of the supplementing a tris-fructose-egg yolk based freezing extender with1 mMand2.5 mMof N-acetylcysteine to sperm recovered from epididymis stored at4ºCfor 24, 48, 72 or 96 hours prior cryopreservation. Motility values and sperm kinematic parameters were compared against control (epididymis stored for 24 hours and no antioxidant addition). Our results showed that N-acetylcysteine addition did not improve sperm motility parameters at any of the time points or dosages tested. In addition, storage of bullfight epididymis up to 96 hours did not significantly affect sperm kinematic parameters or total and progressive motility.

RESUMEN

La raza de Lidia es considerada una insignia de la industria ganadera española. Las tecnologías de reproducción asistida como la criopreservación de espermatozoides epididimarios podría ser considerara como una herramienta importante para obtener más crías y conservar su genética. Ya que estos toros no son seleccionados por su desempeño reproductivo o congelabilidad espermática, la calidad de sus eyaculados es pobre y la adición de antioxidantes antes de la criopreservación podría tener efectos beneficiosos sobre la calidad del semen descongelado. El propósito de este estudio fue evaluar el efecto de la suplementación del medio a base de tris-fructosa y yema de huevo con 1 mM y 2,5 mM de N-acetilcisteína a espermatozoides recuperados de epidídimos almacenados a 4°C por 24, 48, 72 o 96 horas antes de la criopreservación. Los valores de movilidad y los parámetros de cinética espermática fueron comparados con el control (epidídimos almacenados por 24 horas y sin la adición de antioxidantes). Nuestros resultados muestran que la adición de N-acetilcisteína no mejora los parámetros de motilidad espermática en ninguno de los momentos o dosis evaluadas. Además, el almacenamiento de epidídimos refrigerados hasta 96 horas no afecta significativamente los parámetros de cinética espermática o la movilidad total o progresiva. 


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Referencias


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