EFFECTS OF THE LOW INTENSITY CENTRIFUGATION AND THE BREED ON THE QUALITY OF FRESH CANINE SEMEN

Rebeca Marques Mascarenhas, Tarcízio Antonio Rego de Paula

Resumen


The effect of the low intensity centrifugation (300xg), as well as the breed variation were evaluated. To this, semen of 12 dogs of the Beagle (n = 3), Schnauzer (n = 3), Doberman (n = 3) and Boxer (n = 3) breeds, 3 ejaculates collected per individual, totaling 36 ejaculates were used. The direct microscopic evaluation of the motility parameters and the hypoosmotic and supravital staining tests were used. The individual means of sperm vigor and motility varied from 3.5 to 5.0 and from 63 to 90% respectively, while the breed means ranged from 4.4 to 4.9 and from 80 to 90%, respectively. No significant difference was observed in any of the parameters evaluated in fresh or centrifuged semen of dogs among the different breeds studied. Centrifugation of semen resulted in a reduction of about 13% in sperm vigor and 14% in motility and sperm index. The analysis of the parameters of viability and sperm membrane integrity shows a 38% and 14% reduction in the percentage of reactive cells to the hypoosmotic test and supravital staining respectively. These results allow us to conclude that the centrifugation protocol, even at low intensity, significantly reduces sperm motility, the membrane integrity and sperm viability, this effect being more clearly perceived through the hypoosmotic test.

 

RESUMEN

El efecto de la centrifugación a baja intensidad (300xg), así como la variación racial fueron evaluados. Para esto, semen de 12 perros de las razas Beagle (n=3), Schnauzer (n =3), Doberman (n = 3) y Boxer (n = 3), 3 eyaculados colectados por individuo, totalizando 36 eyaculados fueron usados. La medición por microscopia directa de los parámetros de motilidad, así como el test hipoosmótico y la tinción supravital fueron usadas. La media individual del vigor espermático y la motilidad variaron desde 3,5 a 5,0 y desde 63 a 90% respectivamente, mientras que las medias de las razas variaron desde 4,4 a 4,9 y desde 80 a 90%, respectivamente. No se observaron diferencias significativas en los parámetros evaluados el semen fresco y centrifugado entre las razas estudiadas. El análisis de los parámetros de vitalidad y la integridad de la membrana espermática muestra una reducción de 38% y 14% en el porcentaje de células reactivas al test hipoosmótico y a la tinción supravital respectivamente. Estos resultados llevan a la conclusión de que el protocolo de centrifugación, aun a baja intensidad, significativamente reduce la motilidad espermática y la integridad de la membrana y vitalidad espermática, siendo este efecto percibido más claramente a través del test hipoosmótico.


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