CORRELATION BETWEEN CONVENTIONAL SPERM ASSAY PARAMETERS OF FREEZABLE AND NON-FREEZABLE BULL SEMEN

Shriya Gupta, Madhumeet Singh

Resumen


This study investigated correlation between various semen quality parameters for freezable and non-freezable ejaculates. Work was conducted at Livestock Development Board Sperm Station Palampur, Himachal Pradesh, India on semen from six Jersey and two H.F x Sahiwal crossbred bulls. Total 64 ejaculates (8 per bull), were analyzed at four stages of semen processing i.e., post dilution, post equilibration, post thaw and 1hr post thaw incubation at 37ºC for progressive motility, livability, reaction to hypo-osmotic solution, acrosomal integrity and gross morphological abnormalities. Based on these parameters ejaculates were classified into freezable (n=49) and non-freezable (n=15). The percentages mean (±SE) values of each parameter were recorded at post dilution, post equilibration, post thaw and 1hr post thaw incubation for freezable and non-freezable ejaculates, respectively; live sperm (85.16±0.70, 80.51±0.76, 67.92±1.30, 53.33±1.56 vs. 80.80±1.97, 71.87±2.41 56.87±2.50, 42.13±2.17); progressive motility (80.31±0.72, 74.45±0.86, 57.33±1.76, 38.78±1.55 vs. 58.87±4.21, 53.20±4.42, 36.07±2.43, 24.27±1.90); HOST (78.51±1.00, 72.27±1.10, 60.00±1.60, 45.29±1.51 vs. 73.93±2.43, 64.00±2.81, 49.00±2.91, 37.40±1.89), intact acrosome (84.84±0.87, 78.82±1.03, 67.39±1.37, 54.12±1.38 vs. 87.60±1.45, 78.07±1.06, 66.40±2.90, 55.27±3.42) and morphological abnormalities (7.35±0.62, 7.61±0.59, 8.16±0.56, 9.14±0.63 vs. 9.27±1.18, 9.87±0.98, 10.00±1.00, 11.27±1.14). Statistical analysis of the data revealed that live sperm was positively correlated with motility (r= 0.94529, r= 0.77696, p<0.01), HOST (r= 0.88718, r= 0.90490, p<0.01), and intact acrosome (r= 0.61313, r= 0.65386, p<0.01) whereas, motility was negatively correlated with morphological abnormalities (r= -0.29514, r= -0.02852, p<0.01) in both freezable and non-freezable ejaculates, respectively.

RESUMEN

Este estudio investigó la correlación entre varios parámetros de calidad espermática en eyaculados congelables y no congelables. El trabajo se llevó a cabo en Livestock Development Board Sperm Station Palampur, Himachal Pradesh, India, con el semen de seis toros Jersey y dos mestizos H.F. x Sahiwal. Se analizaron un total de 64 eyaculados (8 por toro) en cuatro etapas de procesamiento del semen, es decir, post dilución, post-equilibrio, post-descongelación y 1hr después de la descongelación a 37ºC, para detectar motilidad progresiva, viabilidad, reacción a una solución hipoosmótica, integridad acrosomal y anormalidades morfológicas. En base a estos parámetros, los eyaculados se clasificaron en congelables (n = 49) y no congelables (n = 15). Los porcentajes medios (± SE) de cada parámetro en post-dilución, post-equilibración, post-descongelación y 1hr post-descongelación para los eyaculados congelables y no congelables, respectivamente, fueron los siguientes: espermatozoides vivos (85,16±0,70; 80,51±0,76; 67,92±1,30; y 53,33±1,56 vs. 80,80±1,97; 71,87±2,41 56,87 ± 2,50, 42,13 ± 2,17); motilidad progresiva (80,31 ± 0,72, 74,45 ± 0,86, 57,33 ± 1,76, 38,78 ± 1,55 vs. 58,87 ± 4,21, 53,20 ± 4,42, 36,07 ± 2,43, 24,27 ± 1,90); HOST (78,51 ± 1,00, 72,27 ± 1,10, 60,00 ± 1,60, 45,29 ± 1,51 vs. 73,93 ± 64,74, 64,00 ± 2,81, 49,00 ± 2,91, 37,40 ± 1,89), acrosoma intacto (84,84 ± 0,87, 78,82 ± 1,13, 67,39 ± 1,37 , 54,12 ± 1,38 vs. 87,60 ± 1,45, 78,07 ± 1,06, 66,40 ± 2,90, 55,27 ± 3,42) y anomalías morfológicas (7,35 ± 0,62, 7,61 ± 0,59, 8,16 ± 0,56, 9,14 ± 0,63 vs. 9,27 ± 1,18, 9,87 ± 0,98 , 10,00 ± 1,00, 11,27 ± 1,14). El análisis estadístico de los datos reveló que el porcentaje de espermatozoides vivo se correlaciono positivamente con la motilidad correlaciones positivas y negativas significativas (p<0,01) entre varios parámetros de calidad espermática. EL porcentaje de espermatozoides vivos tuvo una correlación positiva con la motilidad (r= 0,94529, r= 0,77696, p<0,01), HOST (r= 0,88718, r= 0,90490, p<0,01), y con el porcentaje de acrosomas intactos (r= 0,61313, r= 0,65386, p<0,01); mientras que la motilidad se correlacionó negativamente con las anormalidades morfológicas (r= -0,29514, r= -0,02852, p<0.01), tanto en los eyaculados congelables como no congelables, respectivamente.


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