CONCEPTION IN WEST AFRICAN DWARF DOES INSEMINATED WITH NORMAL SALINE DILUTED BUCK SEMEN

Uchechi Daureen Gift Ihejirika, Emmanuel Olubisi Ewuola

Resumen


ABSTRACT

This study investigated fertility response of West African Dwarf (WAD) does artificially inseminated with semen diluted with normal saline and the dilution ratio for achieving an optimum conception. Semen was collected from seven proven adult WAD bucks via electro ejaculation method and pooled. The semen collected was divided into 5 portions and each diluted with normal saline at 1:0 (T1, control), 1:1(T2), 1:2 (T3), 1:3 (T4) and 1:4 (T5) corresponding to five treatments. The treatments were evaluated for motility, livability and concentration. Twenty eight does aged 12-24 months with an average weight of 12.15±1.51kg were allotted to the five treatment groups with unequal replicates in a completely randomised design. Oestrus in the does was synchronised using prostaglandin (PGF2α) (Lutalyse(R) Pharmacia and Upjohn CO. NY). Oestrus was detected at 72 to 96 hours and insemination was carried out at 78th and 90th hours with respective treatments at 0.4 mL of diluted semen through the cervix using insemination gun. Conception was monitored by inspection of those that returned to oestrus and with the use of ultrasound scanner at day 65. Collected data were subjected to descriptive statistics and ANOVA. Results indicated that spermatozoa motility ranged from 81.67±2.87% (T1) to 90.00±5.00% (T4). T1 and T5 had lower motility than other treatments. Spermatozoa concentration ranged from 6.38±0.51x109/mL (T5) to 31.87±2.58x109/mL (T1).The number of motile sperm cells inseminated ranged from 5.33±0.59x109/mL (T5) to 26.05±2.75x109/mL (T1). Total live spermatozoa ranged from 5.94±0.58x109/mL(T5) to 29.48±1.51x109/mL (T1). Does inseminated with semen diluted at ratio 1:1 (T2) had the highest percentage conception, while T1, T4 and T5 had the least percentage conception. The present study showed that conception was enhanced when normal saline was used to dilute goat semen at ratio of 1:1, while dilution up to 1:4 also had a comparable percentage conception with the undiluted semen.

 

RESUMEN

En este estudio se investigó la respuesta a la fertilidad en cabras West African Dwarf  (WAD) que se inseminaron  artificialmente con semen diluido en solución salina normal y la relación de dilución para lograr un nivel de concepción óptimo. El semen se recolectó de siete machos WAD adultos probados, mediante electroeyaculación  y fue mezclado. El semen recolectado se dividió en 5 alícuotas y cada una se diluyó con solución salina normal a 1:0 (T1, control), 1:1 (T2), 1:2 (T3), 1:3 (T4) y 1:4 (T5). ) correspondiente a cinco tratamientos. Los tratamientos fueron evaluados por motilidad, vitalidad y concentración. Se usaron veintiocho cabras, con una edad entre 12 a 24 meses y un peso promedio de 12.15±1.51 kg, para los cinco grupos de tratamientos con réplicas desiguales en un diseño completamente al azar. El celo se sincronizó usando prostaglandina (PGF2α) (Lutalyse(R) Pharmacia y Upjohn CO. NY) y se detectó a las 72 a 96 horas y la inseminación se realizó entre 78 y 90 horas con 0,4 ml de semen diluido y a través del cuello uterino utilizando una pistola de inseminación. La concepción se monitorizó mediante la detección del celo y con el uso de ultrasonografía el día 65. Los datos recopilados se sometieron a estadísticas descriptivas y ANOVA. Los resultados indicaron que la motilidad de los espermatozoides osciló entre 81.67±2.87% (T1) y 90.00±5.00% (T4). T1 y T5 tuvieron menor motilidad que otros tratamientos. La concentración de espermatozoides osciló entre 6.38±0.51x109/mL (T5) y 31.87±2.58x109/mL (T1). El número de espermatozoides mótiles inseminados varió desde 5,33±0,59x109/ml (T5) hasta 26,05 2,75x109/mL (T1). El total de espermatozoides vivos osciló entre 5.94±0.58x109/mL (T5) y 29.48±1.51x109/mL (T1). Las hembras inseminados con semen diluido en una proporción de 1:1 (T2) tuvieron el mayor porcentaje de concepción, mientras que T1, T4 y T5 tuvieron el menor porcentaje de concepción. El presente estudio muestra que la concepción se mejora cuando se utiliza solución salina normal para diluir el semen en una proporción de 1:1, mientras que la dilución 1:4 tuvo una concepción porcentual comparable con el semen sin diluir.


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Referencias


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