EFFECT OF DIFFERENT CONCENTRATIONS OF GLYCEROL IN CRYOPRESERVATION OF GADDI GOAT SEMEN

AMIT SHARMA, PANKAJ SOOD, JITENDRA KUMAR CHAUDHARY

Resumen


Goats have greater importance under small familiar systems in India. The unabated decline in Gaddi males of superior genetic merit due to extensive culling and unplanned breeding (inbreeding) accentuates the importance of conserve superior males and their use for artificial insemination. The available literature is unequivocal about the importance of glycerol during sperm cryopreservation. However, the optimal level seems to vary between species and breeds. The present study aimed to evaluate the effect of different concentrations of glycerol (6, 7, and 8%) on the quality of thawed semen from Gaddi bucks. A total of 180 ejaculates from six Gaddi buck were frozen in Tris citrate extender containing 10% of Egg Yolk with 6, 7, or 8% of glycerol. Sperm quality parameters in thawed semen (morphological abnormalities, sperm viability, progressive motility, and HOST response) were compared; in addition, the percentage of change in thawed semen with respect to fresh was determined. No effect of concentration of glycerol was observed on progressive motility and HOST response. Sperm viability was better in semen frozen at 6 and 8% (45.26±1.32% and 45.10±2.81% respectively) in comparison with 7% of glycerol (34.81±2.78%, P<0.05). Lower sperm morphological abnormalities were observed in semen frozen at 6% of glycerol (7.93±0.28, P<0.05) in comparison with 7% (9.18±0.69%) and 8% (9.90±0.55%). A fertility rate of 41.25% was achieved following AI with semen frozen containing 6% of glycerol. In conclusions, 6% of glycerol was a valid option to cryopreservation of semen from Gaddi buck, resulting in better viability and lower abnormalities. In addition, a good fertility response was observed.


RESUMEN

Las cabras están teniendo mayor importancia en los pequeños sistemas familiares en India. Sin embargo, la disminución deliberada de machos Gaddi de alto merito genético debido a la eliminación excesiva y a los cruzamientos no planificados (consanguinidad) acentúan la importancia de conservar machos superiores y su uso para inseminación artificial. La literatura disponible ha confirmado la importancia del glicerol durante la criopreservación espermática. Sin embargo, el nivel óptimo parece variar entre especies y razas. El propósito de este estudio fue evaluar el efecto de diferentes concentraciones de glicerol (6, 7 y 8%) sobre la calidad del semen descongelado de machos de raza Gaddi. Un total de 180 eyaculados de seis machos fueron congelado en diluyente a base de Tris citrato conteniendo 10% de yema de huevo con 6, 7 u 8% de glicerol. Los parámetros de calidad espermática en el semen descongelado (motilidad progresiva, viabilidad, anomalías morfológicas y respuesta a HOST) fueron comparadas, además el porcentaje de cambio de cada parámetro con respecto al semen fresco fue determinado. No se observó un efecto del nivel de glicerol sobre la motilidad progresiva y la respuesta al HOST. La viabilidad espermática fue mejor en el semen congelado con 6 y8% de glicerol (45,26±1,32% y 45,10±2,81% respectivamente) en comparación con el semen congelado con 7% de glicerol (34,81±2,78%, P<0,05). Menos morfoanomalías fueron observadas en el semen congelado con 6% de glicerol (7,93±0,28, P<0,05) en comparación con el congelado con 7% (9,18±0,69%) y 8% (9,90±0,55%). Una tasa de 41,25% de fertilidad fue alcanzada luego de la inseminación con semen congelado con &% de glicerol. En conclusión, 6% de glicerol es una opción validad para la criopreservación de semen de machos cabríos Gaddi, resultando en una mayor viabilidad y menos morfoanomalías espermáticas. Además, una buena fertilidad fue observada. 


Texto completo:

PDF

Referencias


Bailey JL., Bilaodeau JF., Cormier N. 2000. Semen cryopreservation in domestic animals: a damaging capacitating phenomenon. Journal of Andrololgy 20: 1-7.

Barrea-Compean MH., Purdy PH., Dzakurna JM., Newton GR., Nuti LC. 2005. Cholesterol-loaded cyctodextrin improves post-thaw goat sperm motility. Journal of Animal Sciences 83(1): 153-153.

Biswas D., Bari FY., Shamsuddin M., Rahman MM. 2002. Determination of glycerol percentages for preserving the Black Bengal Buck (Capra hircus) spermatozoa for long time. Pak. Journal of Biological Sciences 5: 715-718.

Bloom E. 1977. Sperm morphology with reference to bull infertility. In: Some papers contributed to the 1st All India Symposium on Animal Reproduction, Punjab Agricultural University, Ludhiana, India, pp. 66-8.

Chahota R., Gupta S., Bhardwaj B., Malik P., Verma S., Sharma M. 2015. Seroprevalence studies on animal Chlamydiosis amongst ruminants I five states of India. Veterinary World 8(1): 72-75.

Deka BC., Rao AR. 1986. Effect of Glycerol Level In Tris-based extender and equilibration period on quality of frozen goat semen. Theriogenology 2: 231-237.

Dogra PK., Thakur YP. 2010. Livestock biodiversity of Himachal Pradesh with special reference to pashmina goats. In: Proceeding of National training on “Production, processingand utilization of pashmina fibre” 1-7 Dec Palampur, H.P, India pp 1-12.

Drobnis ES., Crowe LM., Berger T., Anchodoguy TJ., Overstreet JW., Crowe J.H. 1993. Cold shock damage is due to lipid phase transition in cell membranes: a demonstration using sperm as a model. Journal of Experimental Zoology 265(4): 432-437.

Farshad A., Khalili B., Fazeli P. 2009. The effect of different concentrations of Glycerol and DMSO on the viability of Markhoz Goat spermatozoa during different freezing temperatures steps. Pakistan Journal of Biological Sciences 12: 239-245.

Gacitua H., Arav A. 2005. Successful pregnancies with directional freezing of large volume buck semen. Theriogenology 63: 931–938.

Gangwar C., Kharche SD., Kumar S., Jindal SK. 2016. Cryopreservation of goat semen: status and prospects. Indian Journal of Small Ruminants 22: 1–10.

Guthrie HD., Liu J., Critser JK. 2002. Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biology of Reproduction 67: 1811-1816.

Hafez B., Hafez ESE. 2000. Reproduction in Farm Animals. 7th ed. Lippincott Williams and Wilkens, New York. pp. 509.

Hannock JL. 1951. A staining technique for study of temperature shock in semen. Nature 167, 323-324.

Holt WV. 2000. Basic aspects of frozen storage of semen. Animal Reproduction Science 62: 3–22.

ICAR. 2013. Nutrient Requirements of Sheep, Goats and Rabbits. 3rd ed. ICAR, New Delhi.

Kharche SD., Jindal SK., Priyadarshini R, Kumar S., Goel AK., Ramachandran N., Rout PK. 2013. Fertility following frozen semen artificial insemination in Jamunapari goats. Indian Journal of Animal Sciences 83(10): 1071-1073.

Kulaksiz R., Ari UÇ., Daskin A., Üner AG. 2013. The effect of different Glycerol concentrations on freezability of semen from Angora, Kilis and Saanen goats. Slovakian Journal of Animal Sciences 46: 39-44.

Leboeuf B., Restall B., Salamon S. 2000. Production and storage of goat semen for artificial insemination. Animal Reproduction Science 62:113–141.

Nuti I. 2007. Techniques of artificial insemination in goats. In: Youngquist, R.S. and Threlfall, W.R (eds) Current Therapy in Large Animal Theriogenology. 2nd ed. Sounders-Elsevier, St. Louis, MO. pp. 529-534.

OIE 2008. Quality Standards and Guidelines for Veterinary Laboratories, 2nd ed., OIE, Paris.

Pant HC., Barot LR., Kasiraj R., Misra AK., Prabhakar JH. 2002. Acrosomal integrity of buffalo spermatozoa and its relation to fertility. Indian Journal of Animal Reproduction 23: 110-112.

Purdy PH. 2006. A review on goat sperm cryopreservation. Small Ruminant Research 63: 215-225.

R Core Team 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.

Ranjan R., Goel AK., Ramchandran N., Kharche SD., Jindal SK. 2015. Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen. Indian Journal of Small Ruminant 21(1): 32-36.

Rasad SD., Soeparna, Nurcholiah, Setiawan R., Widyastuti R. 2017. Effect of Glycerol level in two different extenders on post thawed sperm quality of crossbred Etawah goat. Journal of Animal & Veterinary Advances 16(8-12): 87-91.

Rather HA., Islam R., Malik AA., Lone FA. 2016. Addition of antioxidants improves quality of ram spermatozoa during preservation at 40C. Small Ruminant Research 141: 24-28.

Rizal M., Toeliher, MR., Yusuf TL., Purwantara B., Situmorang P. 2013. Quality of Garut frozen semen in various concentrations of Glycerol. Indonesian Journal of Animal & Veterinary Sciences 7: 194-199.

Salamon S., Maxwell WM. 2000. Storage of ram semen. Animal Reproduction Science 62: 77-111.

Sariözkan S., Bucak MN., Tuncer PB., Taşdemir U., Kinet H., Ulutaş PA. 2010. Effects of different extenders and centrifugation/washing on post thaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm. Theriogenology 73, 316-323.

Sharma A., Sood P., Dogra P. 2018. Seminal plasma removal improves cryopreserved semen quality in Gaddi bucks. Indian Journal of Animal Reproduction 39(2): 25-28.

Sikarwar AKS., Ramachandra N., Ranjan R., Gangwar C., Aggarwal JK., 2015. Effect of different levels of glyerol and MSO on freeezability of buck semen. Livestock Research International 3: 71-73.

Sonmez M., Demira E. 2004. The effect of ascorbic acid on the freezability of ram semen diluted with extenders containing different proportions of Glycerol. Turkish Journal of Veterinary & Animal Sciences 28: 893- 899.


Enlaces refback

  • No hay ningún enlace refback.


URL de la licencia: https://creativecommons.org/licenses/by/4.0/

ISSN: 2542-3045

Journal of Veterinary Andrology is a peer-reviewed, No-fee Open-access journal.  

Journal of Veterinary Andrology does not charge any publication fees to authors.

The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions

 

Licencia Creative Commons Atribución 4.0 Internacional.

JOURNAL OF VETERINARY ANDROLOGY esta indexada en/ is indexed in:

Índice y Biblioteca Electrónica de Revistas Venezolanas de Ciencias y Tecnología (REVENCYT). Código RVJ002

International Scientific Indexing (ISI)

Scientific Indexing Services. Journal ID 3410

International Institute of Organized Research (I2OR

Directory of Open Access Scholarly Resources (ROAD)

BASE (Bielefeld Academic Search Engine)

Directory of Research Journals Indexing (DRJI)

Actualidad Iberoamericana

Directory of Open Access Journals (DOAJ)

International Veterinary Information Service (IVIS)

Matriz de Informacion para el Análisis de Revistas (MIAR)

Scientific Journal Impact Factor (SJIF)

CiteFactor Academic Scientific Journals

Latindex

Resultado de imagen para revencyt

  Resultado de imagen para international scientific indexing)

 Resultado de imagen para Scientific Indexing Services 

 

 

 

 

Directory of Research Journals Indexing Logo

 

 International Veterinary Information Service - IVIS 

logo

Resultado de imagen para SJIFactor