EFFECT OF ROYAL JELLY ON QUALITY OF CHILLED SEMEN FROM DAMASCUS BUCK

NURDAN COSKUN CETIN, OGUZ KAAN OGUZ KAAN, CAFER TAYYAR ATES, FIKRET KARACA

Resumen


The objective of this study was to evaluate the effect of supplementation of different concentrations of royal jelly (RJ) in Tris-egg yolk (TEY) and Tris-soybean (TSL) extenders on sperm quality of Damascus buck chilled semen. The ejaculates were collected from five bucks using an electroejaculator twice a week during the reproductive season. Semen samples were pooled and diluted in extenders supplemented with RJ (1%, 0,75%, 0,50%, 0,25%) with a final concentration of 150x106 spermatozoa/mL and stored for 72 hours. The samples were evaluated for sperm quality parameters, including motility, viability, abnormality, membrane integrity, pH, osmolarity. When the RJ doses were compared among each other, 1% RJ concentration caused an increase in the percentage of abnormal and dead spermatozoa in the TSL extender. In the TEY extender, the control and 0.25% RJ concentration groups had the lowest motility and viability values. Generally, the addition of medium and high doses of RJ in the TEY extender group was found to be beneficial to sperm motility and plasma membrane integrity. Although motility was terminated at the 48th hour in the TEY control and 0.25% RJ groups, other trial groups continued. In conclusion, it was determined that the TSL extender was superior to the short-time storage of the goat spermatozoa in the between-groups evaluation, and the RJ supplementation did not achieve any success during the liquid storage at 4°C in the TSL extender.

 

RESUMEN

El objetivo de este estudio fue evaluar el efecto de la suplementación de diferentes concentraciones de jalea real (RJ) en los diluyentes Tris-yema de huevo (TEY) y Tris-soja (TSL) sobre la calidad del semen refrigerado de machos cabríos Damasco. Los eyaculados se recogieron dos veces por semana durante la temporada reproductiva de cinco machos utilizando un electroeyaculador. Las muestras de semen se agruparon y diluyeron en TEY y TSJ suplementados con RJ (1%, 0,75%, 0,50%, 0,25%), a una concentración final de 150x106 espermatozoides/ml y se almacenaron durante 72 horas. Las muestras fueron evaluadas para parámetros de calidad como motilidad, viabilidad, anormalidad, integridad de la membrana, pH y osmolaridad. Cuando las dosis de RJ se compararon entre sí, la concentración de RJ al 1% causó un aumento en el porcentaje de espermatozoides anormales y muertos en el extensor de TSL. En el diluyente TEY, el control y los grupos de concentración de 0,25% RJ tuvieron los valores más bajos de movilidad y viabilidad. En general, se encontró que la adición de dosis medias y altas de RJ en el grupo TEY fue beneficiosa para la motilidad de los espermatozoides y la integridad de la membrana plasmática. Aunque la motilidad se terminó a las 48 horas en el control TEY y en los grupos con 0,25% de RJ, en los otros grupos de prueba la motilidad continuó. En conclusión, se determinó que el diluyente TSL fue superior para el almacenamiento a corto plazo de los espermatozoides de macho cabrío en la evaluación entre grupos, y que la suplementación con RJ no logró ningún éxito durante el almacenamiento de líquido a 4°C en el diluyente TSL.


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