ANTIOXIDANT IMPACT OF Zingiber officinale AND Allium sativum ON RABBIT SEMEN



Oxidative stress can reduce semen quality, thereby resulting in poor reproductive performance of male rabbits. This study was conducted to investigate the antioxidant potentials of dietary Zingiber officinale (Z) and Allium sativum (A) on rabbit semen quality. Twenty-eight male rabbits aged 9-10 months old were randomly allotted to seven diets containing 0 (control), 5, 10, and 15 g of Z, and 5, 10, and 15 g of A per kg diet respectively, and fed the experimental diet for seven weeks. Semen was collected weekly using an artificial vagina and assessed for semen volume (ml), spermatozoa motility (%), sperm concentration (cells/ml), and sperm viability (%). Seminal plasma was separated from the semen by centrifugation for Seminal Total Antioxidant Capacity (STAC) concentration. All data obtained were subjected to Analysis of Variance. The result showed that semen volume was significantly (p<0.05) higher in bucks fed Z15 and A10 than those fed the control diet. Spermatozoa motility was higher in Z5 group. Dietary levels of Z and A had a significant (p<0.05) effect on STAC; the highest value was recorded in bucks fed diet A10 (5.31mol/L). No correlation exists between seminal total antioxidant capacity and semen parameters in the Z and A groups of bucks. The study suggests that supplementation of male rabbits’ diet with Zingiber officinale or Allium sativum at the levels used in this study may not result in an optimum semen quality improvement as parameters assessed varied across the groups. However, dietary Allium sativum at 10g/kg can boost sperm production and antioxidant level in rabbit semen.



El estrés oxidativo puede reducir la calidad del semen, lo que resulta en un pobre desempeño reproductivo en conejos. Este estudio se realizó para investigar el potencial antioxidante del Zingiber officinale (Z) y Allium sativum (A) en la calidad del semen de conejo. Se asignaron al azar veintiocho conejos machos de 9 a 10 meses de edad a siete dietas que contenían 0 (control), 5, 10 y 15 g de Z, y 5, 10 y 15 g de A por kg de dieta, respectivamente, y se alimentaron con las dietas experimentales durante siete semanas. El semen se colectó semanalmente utilizando una vagina artificial y se evaluó el volumen seminal (ml), la motilidad espermática (%), la concentración de espermatozoides (células/ml) y la viabilidad espermática (%). El plasma seminal se separó del semen mediante centrifugación para la determinación de la capacidad antioxidante total del plasma seminal (STAC). Todos los datos obtenidos se sometieron a análisis de varianza. El volumen seminal fue significativamente (p <0.05) mayor en los machos alimentados con Z15 y A10 que aquellos alimentados con la dieta control. La motilidad fue mayor en el grupo Z5. La incorporación de Z y A en la dieta tuvo un efecto significativo (p <0.05) sobre STAC; el valor más alto se registró con la dieta A10 (5,31 mol/L). No existe correlación entre la capacidad antioxidante total del plasma seminal y los parámetros seminales en los grupos Z y A. Este estudio sugiere que la suplementación de la dieta de conejos con Zingiber officinale o Allium sativum en los niveles usados puede no resultar en una mejora óptima de la calidad del semen ya que los parámetros evaluados variaron entre los grupos. Sin embargo, Allium sativum a 10 g/kg puede aumentar la producción de esperma y el nivel de antioxidantes en el semen de conejo.


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