EFFECT OF ETHANOL Moringa oleifera Lam LEAF EXTRACT ON SPERMATOZOA QUALITY OF EXTENDED CAPRINE SEMEN
Resumen
This study was designed to determine the effect of ethanol moringa leaf extract (EMLE) on the spermatozoa quality of extended caprine semen. Semen was collected from three Red Sokoto bucks with the use of an electro-ejaculator. Moringa leaf extract was obtained with ethanol, using 50g of fresh moringa leaves in 300mL of solvent using the soxhlet extraction method. The extract was collected by evaporating the solvent using a rotary evaporator. Ejaculates from the bucks were extended in sodium citrate extender with EMLE at 0 (control), 0.5, 1.0, 1.5, and 2.0 g/L and refrigerated at 5oC for 72hours. Sperm motility, sperm concentration, sperm livability, sperm morphology, and pH were determined every 24 hours. Data were analyzed using analysis of variance. At 72 hours of storage, 2.0 g/L EMLE had the highest motility value (p<0.05); EMLE had no significant effect on sperm concentration throughout storage (p>0.05). At 24, 48, and 72 hours EMLE had no significant (p>0.05) effect on caprine spermatozoa morphology. At 72 hours of storage, 0.5g/L EMLE had the highest pH value (6.36) (p<0.05). In conclusion, EMLE up to 2.0 g/L could be an additive for preserving refrigerated caprine semen for up to 72 hours without compromising the integrity of the sperm cells.
RESUMEN
Este estudio fue diseñado para determinar el efecto del extracto etanólico de la hoja de moringa (EMLE) sobre la calidad de espermatozoides del semen caprinos diluidos. El semen se extrajo de tres machos Red Sokoto con el uso de un electroejaculator. El extracto de hoja de moringa se obtuvo con etanol, mezclando 50 g de hojas frescas en 300 ml de disolvente, mediante el método de extracción soxhlet. El extracto se recogió evaporando el disolvente usando un rotavapor. Los eyaculados de los machos se diluyeron en citrato de sodio con EMLE a 0 (control), 0,5, 1,0, 1,5 y 2,0 g/L y se refrigeraron a 5 °C durante 72 horas. La motilidad, concentración, viabilidad, morfología de los espermatozoides, así como el pH se determinaron a intervalos de 24 horas. Los datos se analizaron mediante análisis de varianza. A las 72 horas de almacenamiento, EMLE 2,0 g/L tuvo el valor de motilidad más alto (p <0,05); la adición de EMLE no tuvo un efecto significativo sobre la concentración espermática durante el almacenamiento (p> 0.05). A las 24, 48 y 72 horas, EMLE no tuvo un efecto significativo (p> 0,05) sobre la morfología espermática. A las 72 horas de almacenamiento, EMLE 0.5 g/L tuvo el valor de pH más alto (6.36) (p <0.05). En conclusión, EMLE hasta 2.0 g/L podría ser un aditivo para conservar el semen caprino refrigerado hasta por 72 horas sin comprometer la integridad de los espermatozoides.
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